![]() ![]() ![]() drug candidate, toxic substance, physical stress,…). Homeostasis status is the first relevant piece of information that can be extracted from cultured live cells subjected to a condition (i.e. A recent summary 1 identified many live cell assays dedicated to measurement and assessment but also pointed out that very few have been used for industrial or, more critically, regulatory purposes. Our data show that intracellular oxidation can be controlled by tuning light energy, opening applications in regulatory purposes, anti-oxidant research, chemotherapy efficacy and dynamic phototherapy strategies.Įxplosion of knowledge in cell biology during the past twenty years has led to the integration of live cell assays into the industry. Reversible modulation of LUCS by both verapamil and proton availability indicated that plasma membrane proton/cation antiporters, possibly of the MATE drug efflux transport family, are involved. Electron Paramagnetic Resonance experiments showed that the singlet oxygen and hydroxyl radical are involved downstream of the light effect, presumably leading to deleterious oxidative stress that massively opens access of the dye to its intracellular target. The mechanisms of the LUCS process were investigated. ![]() Optimization of the LUCS process resulted in a standardized, straightforward and high throughput assay with applications in toxicity assessment. Observations of fluorescent cyanine dye behavior under illumination at 500 nm lead to a novel concept in cell biology allowing the development of a new live cell assay called LUCS, for Light-Up Cell System, measuring homeostasis in live cells. ![]()
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